public sequence databases and analysis services Search Results


barcoded transnetyx microbiome collection tubes 420  (Transnetyx)
97
Transnetyx barcoded transnetyx microbiome collection tubes 420
Barcoded Transnetyx Microbiome Collection Tubes 420, supplied by Transnetyx, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/Microbiome/pm41905514-203-0-1
Average 97 stars, based on 1 article reviews
barcoded transnetyx microbiome collection tubes 420 - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier
trna sequencing service and subsequent statistical analysis  (CloudSeq Biotech Inc)
90
CloudSeq Biotech Inc trna sequencing service and subsequent statistical analysis
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Trna Sequencing Service And Subsequent Statistical Analysis, supplied by CloudSeq Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/trna+sequencing+service+and+subsequent+statistical+analysis/pmc10659772-270-0-10
Average 90 stars, based on 1 article reviews
trna sequencing service and subsequent statistical analysis - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
sequencing services and data analysis  (Igenbio Inc)
90
Igenbio Inc sequencing services and data analysis
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Sequencing Services And Data Analysis, supplied by Igenbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/sequencing+services+and+data+analysis/10__1128_slash_mra__00521___23-37-11-5
Average 90 stars, based on 1 article reviews
sequencing services and data analysis - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
library preparation, sequence analysis, and bioinformatics service  (LC Sciences)
90
LC Sciences library preparation, sequence analysis, and bioinformatics service
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Library Preparation, Sequence Analysis, And Bioinformatics Service, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/library+preparation++sequence+analysis++and+bioinformatics+service/pmc10804318-149-6-11
Average 90 stars, based on 1 article reviews
library preparation, sequence analysis, and bioinformatics service - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
database construction and sequencing services  (MetWare Ltd)
90
MetWare Ltd database construction and sequencing services
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Database Construction And Sequencing Services, supplied by MetWare Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/database+construction+and+sequencing+services/pmc09740276-63-4-9
Average 90 stars, based on 1 article reviews
database construction and sequencing services - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
database construction and sequence analysis  (CloudSeq Biotech Inc)
90
CloudSeq Biotech Inc database construction and sequence analysis
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Database Construction And Sequence Analysis, supplied by CloudSeq Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/database+construction+and+sequence+analysis/pmc09965787-118-4-13
Average 90 stars, based on 1 article reviews
database construction and sequence analysis - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pcr and bi-directional sequence analysis for stx1 and stx2  (Clarient Diagnostic Services Inc)
90
Clarient Diagnostic Services Inc pcr and bi-directional sequence analysis for stx1 and stx2
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Pcr And Bi Directional Sequence Analysis For Stx1 And Stx2, supplied by Clarient Diagnostic Services Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/pcr+and+bi+directional+sequence+analysis+for+stx1+and+stx2/10__1128_slash_jcm__01939___16-33-21-23
Average 90 stars, based on 1 article reviews
pcr and bi-directional sequence analysis for stx1 and stx2 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
whole-genome sequencing and comprehensive analysis services  (Knome Inc)
90
Knome Inc whole-genome sequencing and comprehensive analysis services
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Whole Genome Sequencing And Comprehensive Analysis Services, supplied by Knome Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/whole+genome+sequencing+and+comprehensive+analysis+services/pm19488835-57-6-0
Average 90 stars, based on 1 article reviews
whole-genome sequencing and comprehensive analysis services - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
comprehensive and public sequence database for identification of mycobacterium species  (Ridom GmbH)
90
Ridom GmbH comprehensive and public sequence database for identification of mycobacterium species
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Comprehensive And Public Sequence Database For Identification Of Mycobacterium Species, supplied by Ridom GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/comprehensive+and+public+sequence+database+for+identification+of+mycobacterium+species/10__48165_slash_ijvsbt__18__5__17-96-9-0
Average 90 stars, based on 1 article reviews
comprehensive and public sequence database for identification of mycobacterium species - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
sequencing services for microbiota analysis of patients with ibd and ibs  (Genetic Analysis AS)
90
Genetic Analysis AS sequencing services for microbiota analysis of patients with ibd and ibs
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Sequencing Services For Microbiota Analysis Of Patients With Ibd And Ibs, supplied by Genetic Analysis AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/sequencing+services+for+microbiota+analysis+of+patients+with+ibd+and+ibs/10__1016_slash_j__clinmicnews__2019__03__005-94-26-0
Average 90 stars, based on 1 article reviews
sequencing services for microbiota analysis of patients with ibd and ibs - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
databases and algorithms for sequence analysis  (Congenica)
90
Congenica databases and algorithms for sequence analysis
The deletion of cytoplasmic <t>tRNA</t> methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite <t>sequencing</t> of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.
Databases And Algorithms For Sequence Analysis, supplied by Congenica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/public+sequence+databases+and+analysis+services/databases+and+algorithms+for+sequence+analysis/10__1089_slash_clinomi__06__05__11-51-17-0
Average 90 stars, based on 1 article reviews
databases and algorithms for sequence analysis - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


The deletion of cytoplasmic tRNA methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite sequencing of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.

Journal: Clinical and Translational Medicine

Article Title: The m 5 C methyltransferase NSUN2 promotes codon‐dependent oncogenic translation by stabilising tRNA in anaplastic thyroid cancer

doi: 10.1002/ctm2.1466

Figure Lengend Snippet: The deletion of cytoplasmic tRNA methylation induced by NSUN2 knockdown showed a species bias and secondary structure bias, and led to a decrease in the overall protein translation level. (A and B) LC–MS analysis of total 5‐methylcytidine (m 5 C) levels in purified tRNA from vector (A) and shN (B) cells. (C) Heatmap of m 5 C‐modified cytoplasmic tRNAs in the NSUN2 knockdown and control cells. Each cell shows the summarised m 5 C level of a representative tRNA isodecoder. The colour represents the relative intensity of methylation changes. (D) Heatmap illustrating the methylation levels of different cytosines on candidate tRNAs, as determined by bisulphite sequencing of tRNA preparations from the NSUN2 knockdown and control cells. A gradient from green to red indicates the methylation rate from 0 to 100%. (E) Quantification of m 5 C level on m 5 C‐modified tRNAs. (F) Motif sequence at m 5 C sites, p value < .001, E value < 0.01. (G) Ridge plot showing the change of m 5 C methylation rate density on tRNA secondary structure between vector and shN cells. Each short vertical line represents methylation rate of a specific m 5 C site. (H) The effects of NSUN2 knockdown and expression on protein synthesis in KHM‐5 M, BHT‐101 and 8305C as analysed by puromycin intake assay. (I) Statistical analysis on the basis of puromycin incorporation to monitor protein synthesis. Data are shown as mean ± SD of N = 3 biological replicates. (J) Human genome mature tRNA Leu‐CAA and CAG, the green part indicates that there are detectable m 5 C modification sites on the tRNA sequence.

Article Snippet: tRNA sequencing service and subsequent statistical analysis were provided by CloudSeq Inc. ( ).

Techniques: Methylation, Knockdown, Liquid Chromatography with Mass Spectroscopy, Purification, Plasmid Preparation, Modification, Control, Bisulfite Sequencing, Sequencing, Expressing

Depletion of NSUN2‐mediated m5C caused a decrease in tRNA and an increase in cleaved fragments. (A) A method for tRNA charging assay. (B) tRNA charging level of indicated isodecoder family was measured. Primers specific to Leu‐CAA and Leu‐CAG were used. (C) qRT‐PCR analysis of tRNA Leu‐CAA. (D) Relationship of tRNA related RNA fragments. (E) qRT‐PCR analysis of tRNA Leu related RNA fragments. (F) Correlation scatter plot showing that tRNA expression is positively associated with m 5 C modification at variable loop. (G) Comparison between tRNA Leu‐CAA isodecoders expression and m 5 C modification. The bubble size represents the proportion of tRNA Leu‐CAA isodecoders in vector cell. (H) Comparison between tRNA expression and m 5 C modification at variable loop. Each row represents the same tRNA isodecoder. (I) The 3D structure diagram shows the position of C48 (green dot) and TΨC loop/arm (red shaded part) in tRNA Leu‐CAA‐2‐1. The blue, red, white orange and grey sphere represents the nitrogen atom ( N ), oxygen atom ( O ), hydrogen atom ( H ), phosphorus atom ( P ) and the carbon atom ( C ). In the secondary structure of tRNA Leu‐CAA, short red line indicates the possible cleavage sites of tRNA after methylation deletion. (J) Working model for NSUN2 mediated tRNA m 5 C modification in regulation of ATC tumourigenesis. NSUN2 sustains tRNA stability by catalysing m 5 C modification and enhances translation in cancer.

Journal: Clinical and Translational Medicine

Article Title: The m 5 C methyltransferase NSUN2 promotes codon‐dependent oncogenic translation by stabilising tRNA in anaplastic thyroid cancer

doi: 10.1002/ctm2.1466

Figure Lengend Snippet: Depletion of NSUN2‐mediated m5C caused a decrease in tRNA and an increase in cleaved fragments. (A) A method for tRNA charging assay. (B) tRNA charging level of indicated isodecoder family was measured. Primers specific to Leu‐CAA and Leu‐CAG were used. (C) qRT‐PCR analysis of tRNA Leu‐CAA. (D) Relationship of tRNA related RNA fragments. (E) qRT‐PCR analysis of tRNA Leu related RNA fragments. (F) Correlation scatter plot showing that tRNA expression is positively associated with m 5 C modification at variable loop. (G) Comparison between tRNA Leu‐CAA isodecoders expression and m 5 C modification. The bubble size represents the proportion of tRNA Leu‐CAA isodecoders in vector cell. (H) Comparison between tRNA expression and m 5 C modification at variable loop. Each row represents the same tRNA isodecoder. (I) The 3D structure diagram shows the position of C48 (green dot) and TΨC loop/arm (red shaded part) in tRNA Leu‐CAA‐2‐1. The blue, red, white orange and grey sphere represents the nitrogen atom ( N ), oxygen atom ( O ), hydrogen atom ( H ), phosphorus atom ( P ) and the carbon atom ( C ). In the secondary structure of tRNA Leu‐CAA, short red line indicates the possible cleavage sites of tRNA after methylation deletion. (J) Working model for NSUN2 mediated tRNA m 5 C modification in regulation of ATC tumourigenesis. NSUN2 sustains tRNA stability by catalysing m 5 C modification and enhances translation in cancer.

Article Snippet: tRNA sequencing service and subsequent statistical analysis were provided by CloudSeq Inc. ( ).

Techniques: Quantitative RT-PCR, Expressing, Modification, Comparison, Plasmid Preparation, Methylation